The graph below shows the performance of TG40 System. Initially the TG40 microplate is connected to the TG40 docking station and running off an external power supply. The temperatures are programmed using the TG40 Control Centre software running on a PC to maintain the temperature of rows 1 to 5 at temperatures of 20.0°C, 16.0°C, 12.0°C, 8.0°C and 4.0°C respectively.
The TG40 Microplate reaches the set temperatures in 3 minutes then holds these temperatures for 25 hours, when a temperature ramp is programmed to run, increasing the temperature of each row by 6°C. Thi sis then followed by another increasing temperature ramp and finally a ramp of decreasing tremperature.
After 30 minutes the plate is removed from the docking station and continues temperature controlled operation using the built-in battery. The switch between power sources has no effect on the accuracy and stability of the temperature control system. TG40 is also able to control temperature accurately even when the set temperature is close to the ambient temperature (i.e. Row 2 and Row 3).
Temperature Optimization with TG40System
Shown below are the results of optimizing the temperature to obtain protein crystals. In this experiment crystals were obtained at 6°C only and the other temperatures yielded precipitates.
This work was done by Andrew Bent, a protein crystallization scientist scientist at a major pharmaceutical company.